THIN LAYER CHROMATOGRAPH

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The visual presentation of the complete chromatogram showing all samples and standards side by side is one of the most convincing arguments for Thin-Layer Chromatography. No other chromatographic technique can directly express the result as a color image and make it available for visual evaluation.

For electronic image acquisition the camera – like the human eye – captures polychromatic visible light. Under white light illumination it is the light reflected from the layer background. Under long-wavelength UV light (366 nm) it is the light emitted by fluorescent substances. When short-wavelength UV light (254 nm) is used, substances absorbing UV 254 appear as dark zones, provided the layer contains a fluorescence indicator (fluorescence quenching).

Key features

• Reproducible high-quality images acquired under homogeneous illumination with the selected light
• Easy and intuitive operation with visionCATS
• High-dynamic-range imaging (HDRI)
• Side by side comparison of tracks originating from the same or different plates and/or different illumination modes
• Various image enhancement tools, e.g. Spot Amplification (“Spot Amp”), Clean Plate Correction and Exposure Normalization
• Image-based profile generation from reference and sample tracks, and subsequent peak integration and calibration
• New digital CCD camera with a maximum resolution of 82 μm on the plate
• USB 3.0 for easy PC connection
• Meets all requirements to be used in a cGMP/cGLP environment
• IQ/OQ qualification and 21 CFR Part 11 ready